Figure 4 | Cell Death & Disease

Figure 4

From: Fgf9 inhibition of meiotic differentiation in spermatogonia is mediated by Erk-dependent activation of Nodal-Smad2/3 signaling and is antagonized by Kit Ligand

Figure 4

Fgf9-mediated meiotic inhibition in Kit+ spermatogonia is mediated by activation of Nodal signaling. (a) Quantitative RT-PCR analysis of Cripto and Nodal mRNA expression in untreated Kit+ spermatogonia and in the same cells treated overnight with Kl or Fgf9. Data represent the mean±S.D. from five independent experiments. (b) Representative western blot analysis of Smad2 phosphorylation and Cripto expression in Kit+ control spermatogonia and in the same cells treated overnight with Fgf9. (c) Quantitative RT-PCR analysis of Cripto mRNA expression in untreated Kit+ spermatogonia and in the same cells treated overnight with Kl or with Fgf9, in the presence or absence of the Alk4/7 selective inhibitor SB431542. Data represent the mean±S.D. from four independent experiments. (d) Quantitative RT-PCR analysis of Nodal mRNA expression in untreated Kit+ spermatogonia and in the same cells treated overnight the Alk4/7 selective inhibitor SB431542. Data represent the mean±S.D. from three independent experiments. (e) Western blot analysis of Smad2 phosphorylation and expression of meiotic markers (Stra8 and Scp3) in cultured untreated Kit+ spermatogonia and in the same cells treated overnight with Fgf9, in the presence or absence of the Alk4/7 selective inhibitor SB431542. Densitometric analysis of western blots from four independent experiments is shown on the right. Bars represent the mean±S.D.

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