Figure 1
Klf4 and Klf5 are differentially expressed and positively correlated in GEMMs of breast cancer. (a) Microarray analysis of Klf4/5 levels across GEMMs of breast cancer. Data for 58 mammary tumors from the Gene Expression Omnibus (GSE3165) were organized by GEMM and molecular subtype. Expression values were normalized to whole mouse RNA (bars, S.D.). Klf4 levels in luminal and non-luminal tumors were compared via one-way ANOVA using Dunnet’s post test (P<0.0001). (b) Spearman’s correlation was performed for the samples in panel a. (c) qRT-PCR analysis of total RNA isolated from tumors of MMTV-Neu or C3(1) TAg transgenic mice (left panel). Normal mammary tissue from FVB/N mice was analyzed similarly (NL breast, N=3). Tumor mean expression is depicted relative to the mean for normal tissue (bars, S.E.). The overall mean tumor expression of Klf4 and Klf5 was compared between GEMMs using a two-tailed t-test (for each gene, P<0.0001). The log2 transformed data were assessed by Spearman’s correlation (right panel). (d) Western blot analysis of KLF4/5 levels in whole-cell lysate of 10 different breast cancer cell lines. KLF expression was determined using ImageJ and normalized to β-actin. The expression values were assessed by Spearman’s correlation (right panel)
