Figure 1
LincRNA-p21 (Linc-p21) transcripts are highly inducible by UVB in mouse and human keratinocytes in culture and in mouse skin. (a) Balb/MK2 mouse keratinocytes were exposed to UVB, collected 8 h later and immunoblot analysis conducted. (b) Balb/MK2 mouse keratinocytes were exposed to the indicated doses of UVB, collected 16 h later and lincRNA-p21 transcript levels measured. (c) Balb/MK2 cells were exposed to 10 mJ/cm2 UVB and lincRNA-p21 transcript levels measured at the indicated times. (d) NHEK cells were exposed to the indicated doses of UVB, collected 16 h later and lincRNA-p21 transcripts measured. (e) NHEK cells were exposed to 10 mJ/cm2 UVB and lincRNA-p21 transcripts measured at the indicated times. (f) SKH-1 mice (three mice per time point) were exposed to 100 or 200 mJ/cm2 UVB, epidermis was collected at 9 h and lincRNA-p21 levels measured. (g) SKH-1 mice (three mice per group) were treated with 100 mJ/cm2 and lincRNA-p21 transcripts measured at the indicated times. LincRNA-p21 levels were measured by TaqMan real-time PCR (Ct value at peak post UVB=26–29 cycles using 25 ng template for all experiments). LincRNA-p21 was normalized to β-actin. Data are expressed as the mean ±S.D. N≥3, *P <0.05 significantly different compared with time 0 using student t-test
