Figure 3
LincRNA-p21 (linc-p21) does not regulate cell proliferation in UVB-treated keratinocytes. (a) Balb/MK2 cells were transfected with siRNA to lincRNA-p21 or control siRNA, and 48 h post transfection, cells were exposed to 10 mJ/cm2 UVB, collected 18 h later and lincRNA-p21 transcripts measured. (b) Control siRNA and lincRNA-p21 #1 knockdown Balb/MK2 cells were counted at 0, 24 and 48 h post transfection. (c) Control and lincRNA-p21 #1 knockdown Balb/MK2 cells were exposed to 10 mJ/cm2 UVB (48 h post transfection, t=0), and cells were counted 24 and 48 h after UVB (N=3 for each time point). (d) Control and lincRNA-p21 #1 knockdown Balb/MK2 cells were exposed to 10 mJ/cm2 UVB (48 h post transfection) and collected 18 h after UVB and immunoblot analysis conducted. (e) Control and lincRNA-p21 #1 knockdown Balb/MK2 cells were exposed to 10 mJ/cm2 UVB (48 h post transfection) and collected 24 h after UVB, PI-stained and analyzed by FACS. (f) Control and lincRNA-p21 #1 and #2 knockdown Balb/MK2 cells were exposed to 10 mJ/cm2 UVB (48 h post transfection) and pulse-labeled with BrdU for 1 h and collected 6 h after UVB. Cells were stained with BrdU-FITC and PI-stained and analyzed by FACS. Data expressed in a, b, c, e and f as the mean ±S.D. N ≥3, *P<0.05 significantly different compared with UVB-exposed control as determined by the student t-test
