Figure 4
LincRNA-p21 (linc-p21) regulates apoptosis in UVB-treated keratinocytes. (a) Control and lincRNA-p21 #1 knockdown Balb/MK2 cells were exposed to 10 mJ/cm2 UVB (48 h post transfection) and collected at 0, 8 and 18 h after UVB exposure and immunoblot analysis for caspase 3 conducted; results shown are representative of duplicate experiments. (b) Control and lincRNA-p21 #1 knockdown Balb/MK2 cells were exposed to 10 mJ/cm2 UVB (48 h post transfection) and collected at 0, 6, 12, 18 and 24 h after UVB. Cells were stained with annexin-V and PI and analyzed by FACS. Similar results were obtained in three independent experiments. (c) Control and lincRNA-p21 #1 and #2 knockdown Balb/MK2 cells were exposed to 10 mJ/cm2 UVB (48 h post transfection) and 24 h after UVB. Cells were stained with annexin-V and PI and analyzed by FACS. Similar results were obtained in three independent experiments. (d) Control and lincRNA-p21 #1 and #2 knockdown Balb/MK2 cells were exposed to 10 mJ/cm2 and collected at 18 h post UVB and candidate gene expression examined by Taqman RT-PCR. (e) Control and lincRNA-p21 #1 and #2 knockdown Balb/MK2 cells were exposed to 10 mJ/cm2 and collected at 18 h post UVB and immunoblot analysis conducted. (f) NHEK cells were transfected with siRNA to human lincRNA-p21 or control siRNA, 48 h later exposed to 10 mJ/cm2 UVB, collected 18 h later and lincRNA-p21 levels measured. (g) Control and lincRNA-p21 knockdown NHEK cells were exposed to 10 mJ/cm2 UVB (48 h post transfection) and collected at 0 and 18 h after UVB. Cells were stained with annexin-V and PI and analyzed by FACS. Similar results were obtained in three independent experiments. (h) Control and lincRNA-p21 knockdown NHEK cells were exposed to 10 mJ/cm2 and collected at 18 h post UVB, and candidate gene expression examined. Data are expressed in c, d, f, g and h as the mean ±S.D. N ≥3, *P<0.05 significantly different compared with siRNA control as determined by the student t-test
