Figure 5
Mutation of single p53 allele has a dominant-negative effect in vivo in mouse epidermis. (a) K5Cre+/tg, K5Cre+/tg;LSLp53+/R172H, K5Cre+/tg;p53+/flox and K5Cre+/tg;p53flox/flox SKH-1 mice were treated with 200 mJ/cm2 UVB and epidermal lincRNA-p21 transcripts measured 9 h post UVB treatment. (b) K5Cre+/tg, K5Cre+/tg;LSLp53+/R172H, K5Cre+/tg;p53+/flox and K5Cre+/tg;p53flox/flox SKH-1 mice were treated with 100 mJ/cm2 UVB and the number of apoptotic interfollicular basal epidermal keratinocytes/cm skin were scored at 9 h post UVB treatment. Data are expressed as the mean ±S.D. N≥3, *P <0.05 significantly different compared with UVB-treated K5Cre mice as determined by the student t-test
