Figure 5 | Cell Death & Disease

Figure 5

From: Myocardial MiR-30 downregulation triggered by doxorubicin drives alterations in β-adrenergic signaling and enhances apoptosis

Figure 5

High miR-30 levels are protective against DOX insult and reduce ROS generation. (a) Left graph: caspase activity in cardiac cultures treated with DOX alone (1 μmol/l, 18 h) and in combination with miR-30e overexpression through pre-30e mimic transfection (20 nmol/l). Right graph: relative caspase activity upon miR-30 family inhibition using the pEGFP-sp30 sponge vector. (b) Bax and Bcl-2 protein levels measured by western blot analysis. Cells were treated with DOX (1 μmol/l, 6 h and 18 h) and also transfected with pre-30e mimics (20 nmol/l) in combination with DOX (1 μmol/l, 18 h). Band densitometry was measured using Image J. All values are normalized to Tubulin and expressed as Bax/Bcl-2 ratio. (c) ROS generation of transfected cultures for miR-30 overexpression or inhibition, measured by DCFDA fluorescence intensity. All assays (a, b, c) were performed on H9c2 cells and results are average of independent triplicates ±S.E.M. (t-test; ns, not significant)

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