Figure 6 | Cell Death & Disease

Figure 6

From: Myocardial MiR-30 downregulation triggered by doxorubicin drives alterations in β-adrenergic signaling and enhances apoptosis

Figure 6

GATA-6 mediates miR-30 downregulation triggered by DOX. (a) RT-qPCR data showing GATA-6 and miR-30e expression levels in response to DOX time-course treatment (1 μmol/l). (b) Relative levels of primary and mature miR-30d and miR-30e, upon GATA-6 silencing detected by RT-qPCR. (c) RT-qPCR data for miR-30 target mRNA levels in response to GATA-6 silencing (25 nmol/l, 72 h) and after co-transfection with compensatory LNA miR-30 family inhibitors (100 nmol/l, 72 h). (d) Relative caspase activity in response to GATA-6 silencing, ±DOX treatment (1 μmol/l, 18 h). All values (a, b, c, d) were obtained performing biological triplicates on H9c2 cells and are presented as averaged ratio to the respective control±S.E.M. All RT-qPCR results were normalized to the correspondent U6 Ct value (ns, not significant; t-test)

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