Figure 5 | Cell Death & Disease

Figure 5

From: Activation of NRG1-ERBB4 signaling potentiates mesenchymal stem cell-mediated myocardial repairs following myocardial infarction

Figure 5Figure 5

Conditioned medium of MSC-ERBB4 protects cardiomyocytes. (a) The conditioned medium of MSC-ERBB4 promoted cardiomyocyte growth, which was blunted by anti-NRG1 antibody. (b) More Ki67pos cells were found in α-actininpos cardiomyocytes cultured with conditioned medium of MSC-ERBB4 than that of MSCe, indicating a more prosper cell dividing. The number of Ki67pos cells declined when anti-NRG1 antibody was applied into conditioned medium of MSC-ERBB4. Scale bar=200 μm. (c) The conditioned medium of MSC-ERBB4 prevented cardiomyocytes from senescence, which was erased by anti-NRG1 antibody. (d) The conditioned medium of MSC-ERBB4 prevented cardiomyocytes from apoptosis by enhancing p-Akt and Bcl-2 expression. DAPI, 4',6-diamidino-2-phenylindole; CM, conditioned medium; Bcl-2, B-cell lymphoma 2; p-Akt, phosphorylated Akt; t-Akt, total Akt; anti-NRG1, anti-NRG1 antibody

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