Figure 2 | Cell Death & Disease

Figure 2

From: The synergistic interaction between the calcineurin B subunit and IFN-γ enhances macrophage antitumor activity

Figure 2

CnB synergizes with IFN-γ to polarize TAMs to M1-like phenotype in vitro. (a) Identification of TAMs’ purity. P2 represents the percentage of F4/80-positive cells. Filled histogram represents the fluorescence of F4/80 antibody staining, and open histogram represents the fluorescence of isotype antibody staining. (b–g) Quantitative PCR analysis of M1 and M2 markers’ expression in drugs-stimulated TAMs. Duration of drug treatments, 10 h. b: IL-12; c: CD86; d: TRAIL; e: CXCL9; f: CXCL10; g: CD206. (h and i) Enzyme-linked immunosorbent assay of IL-12 and CXCL9 production (time course). h: IL-12; i: CXCL9. In panels h and i, the t-tests were carried out between CnB+IFN-γ treatment and IFN-γ treatment alone. *P<0.05, **P<0.01, ***P<0.001

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