Figure 3 | Cell Death & Disease

Figure 3

From: The synergistic interaction between the calcineurin B subunit and IFN-γ enhances macrophage antitumor activity

Figure 3

CnB synergizes with IFN-γ to skew human monocyte-derived macrophages to an M1-like phenotype. (a–d) Quantitative PCR assay of the synergistic effect of CnB and IFN-γ in inducing some M1 and M2 marker transcripts. a: IL-12; b: TRAIL; c: ARG1; d: CD206. (e and f) Enzyme-linked immunosorbent assay of TRAIL and IL-12 production upon CnB and IFN-γ stimulation. e: TRAIL; f: IL-12. (g) Flow cytometry analysis of CD206 expression on PBMC-derived macrophages. Cell were treated with CnB (20 μg/ml), IFN-γ (3 ng/ml), or CnB (20 μg/ml)+IFN-γ (3 ng/ml) for 24 h. P1, percentage of CD206-negative cells; P2, percentage of CD206-positive cells; Green: isotype control; Black: medium control; Blue: CnB; Red: IFN-γ; Gray: CnB+IFN-γ. In panels e and f, the t-tests were carried out between CnB+IFN-γ treatment and IFN-γ treatment alone. *P<0.05, **P<0.01, ***P<0.001

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