Figure 7
Reciprocal repression between XIST and miR-92b in HCC cells. (a) Diagram of the two miR-92b putative binding sites and corresponding mutant sites in XIST mRNA sequences. (b) Effects of miR-92b on the expression of reporter genes containing XIST-1 or XIST-2 sequences. Each luciferase activity was normalized to the value obtained in the cells transfected with NC mimics. (c) Relative miR-92b (left panel) or XIST expression (right panel) of QSG-7701 and QGY-7703 cells were analyzed by qRT-PCR. The data were normalized against the expression level of miR-92b or XIST in QSG-7701 cells. (d) Relative miR-92b expression in QGY-7703 cells transfected with XIST siRNA or NC siRNA were analyzed by qRT-PCR. The data were normalized to the expression level of miR-92b in the cells transfected with NC siRNA. (e) Relative XIST expression in QGY-7703 cells transfected with miR-92b or NC mimics were analyzed by qRT-PCR. The data were normalized to the expression level of XIST in the cells transfected with NC mimics. Results were represented as mean±S.D. (n=3). (f) Expression of XIST in 28 pairs of female HCC tissues and ANLTs were determined by qRT-PCR. The expression levels were compared with paired Student’s t-test. (g) The negative correlation between XIST and miR-92b expression in female HCC tissues (r=−0.5332, ***P=0.0035, n=28)
