Figure 4
CTB transport in the proximal portion of the optic nerve is not affected by VEGF-A neutralization. (a) Tile scan confocal images of longitudinal sections of the entire optic nerve, between the posterior globe and optic chiasm, were used to quantify CTB anterograde transport along the optic nerve (top panel). Measurements of fluorescence intensity were taken at 100 μm intervals (bottom panel; white vertical lines representing 100 μm), plotted over the length of the tissue (b); data from 48 h post-CTB injection time point, and used to calculate the area under the curve (c). AUC fluorescence intensity increases with time post-CTB injection; however, no significant differences were observed at any of the time points between IgG1 and sVEGFR-2-treatment groups. For presentation purposes, only data points every 500 μm are shown in (b); however, AUC was calculated from 100 μm intervals along the optic nerve. N=3–10 optic nerves. Data are given as means±S.E.M. Original magnification= × 4
