Figure 1 | Cell Death & Disease

Figure 1

From: Guttiferone K impedes cell cycle re-entry of quiescent prostate cancer cells via stabilization of FBXW7 and subsequent c-MYC degradation

Figure 1

GUTK inhibited DNA synthesis following induction of cell cycle re-entry in prostate cancer cells. Chemical structure of GUTK (a). LNCaP (b) and PC-3 (c) cells was synchronized at quiescence by serum withdrawal for 7 days or contact inhibition for 3 days, before being induced to re-enter the cell cycle by serum restoration or re-plating at low density, respectively. GUTK at indicated concentrations was introduced at the time of induction and the cells were harvested and assessed for DNA content using the SYBR Green assay at 24, 48 and 72 h. Data are shown as the mean±S.D. of three independent experiments, *P<0.05, **P<0.01, ***P<0.001 versus control. Control cells (DMSO) were induced to re-enter the cell cycle in DMSO-containing medium without GUTK. Quiescent cells were analyzed to show DNA content prior to induction of cell cycle re-entry

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