Figure 1
From: Myt3 suppression sensitizes islet cells to high glucose-induced cell death via Bim induction
Myt3 suppression impairs marginal but not optimal islet graft function. (a) Random-fed blood glucose measurements for mice transplanted with an optimal mass (300 islets) of shScramble- or shMyt3-transduced islets. Results are represented as mean±S.E.M. of seven independent experiments. (b) Area under the curve for blood glucose measurements from day 1 to 35 post-optimal transplant. Results are represented as mean±S.E.M. of seven independent experiments. (c) Random-fed blood glucose measurements for mice transplanted with a marginal mass (150 islets) of shScramble- or shMyt3-transduced islets. Results are represented as mean±S.E.M. of five independent experiments. *P<0.05, **P<0.01, ***P<0.001 comparing mice receiving grafts using shMyt3-transduced islets with mice receiving grafts using shScramble- transduced islets (two-way ANOVA). (d) Area under the curve for blood glucose measurements from day 1 to 35 post-suboptimal transplant. Results are represented as mean±S.E.M. of five independent experiments. ***P<0.001 comparing mice receiving grafts using shMyt3-transduced islets to mice receiving grafts using shScramble- transduced islets (unpaired t-test). (e) Kaplan–Meier survival curve comparing time to return to normoglycaemia for mice receiving marginal mass transplants of shScramble- or shMyt3-transduced islets. Mice were considered non-diabetic after two consecutive blood glucose measurements <12 mM
