Figure 6
From: Myt3 suppression sensitizes islet cells to high glucose-induced cell death via Bim induction
Myt3 suppression sensitizes islet cells to high glucose-induced cell death via upregulation of Bim. (a) Bim protein was determined by western blot in shScramble- or shMyt3-transduced islets under normal glucose (NG) or high glucose (HG) conditions. (b) Quantification of Bim protein as a fraction of Gapdh protein in (a). Results are represented as mean±S.E.M. of three independent experiments. *P<0.05, **P<0.01, ***P<0.001 comparing the indicated bars (two-way ANOVA). (c) qPCR analysis of Bim expression in dispersed islet cells transduced with shScramble (L-shScram) or shBim (L-shBim) expressing lentiviruses. Results are represented as mean±S.E.M. of four independent experiments. ***P<0.001 comparing L-shScram to L-shBim transduced cells (unpaired t-test). (d) Cell death was determined by propidium iodide incorporation in dispersed islet cells co-transduced with lentiviral shScramble (L-shScram) or shBim (L-shBim), and adenoviral shScramble (Ad-shScram) or shMyt3 (Ad-shMyt3), under high glucose conditions. Results are represented as mean±S.E.M. of four independent experiments. ***P<0.001 comparing the indicated bars (two-way ANOVA)
