Figure 4

Aberrant immune response of miR-155-KO skeletal muscle after injury. (a) Immunostaining for CD11b (green) and DAPI (red) on transverse sections of miR-155-WT and miR-155-KO TA muscles 1 day after CTX injury. Bar, 50 μm. (b) Immunostaining for F4/80 or CD11b (green) and DAPI (red) on transverse sections of miR-155-WT and miR-155-KO TA muscles 3 days after CTX injury. Bar, 100 μm. (c) Real-time PCR for expression of CD11b, CD68, TNFα, IL-6, MCP-1, INFγ, RIP3, and IL-10 in miR-155-WT and miR-155-KO TA muscles 1, 3, and 7 days after CTX injury. Data are quantified from six animals for each genotype and are presented as Mean±S.E.M. *P<0.05. (d) Relative numbers of inflammatory cells (F4/80- and CD11b-positive cells isolated using MACS) from miR-155-WT (black circle) and miR-155-KO (red triangle) TA muscles 1, 3, 5, and 7 days after CTX injury. Four to six animals of each genotype were quantified and data are presented as Mean±S.E.M. (e) Real-time PCR for expression of iNOS, CD68, CD206, IL-6, TNFα, and MCP-1 in MACS-isolated macrophages from miR-155-WT and miR-155-KO TA muscles 3 days after CTX injury. Four to six animals of each genotype were quantified and data are presented as Mean±S.E.M. *P<0.05