Figure 7
Overexpression of miR-155 mimic in TA muscle after CTX injury rescues muscle regeneration defects in miR-155-KO mice. (a) H&E staining of transverse sections of TA muscle from miR-155-KO with or without miR-155 mimic injection at 3, 7, 14, and 21 days after CTX injury. Bar, 100 μm. (b) miR-155 expression was measured by Taqman real-time PCR assays 3 days after CTX injury and miRNA mimic injection. Four to six animals in each condition were analyzed and data are presented as Mean±S.E.M. *P<0.05. (c) SOCS1 mRNA levels were measured by real-time PCR in TA muscle of control mice and miR-155-KO mice injected with miRNA control mimic or miR-155 mimic 3 days after CTX injury. Injection of miR-155 mimic effectively inhibits SOCS1 levels in miR-155-KO mice. Four to six animals in each condition were analyzed and data are presented as Mean±S.E.M. *P<0.05. (d) Real-time PCR quantification of inflammatory markers (TNFα, MCP-1, CD68, IL-6) that are affected in miR-155-KO mice after CTX injury, showing restoration of these delayed macrophage activation markers after miR-155 mimic injection. (e) Real-time PCR shows that delayed eMHC and myogenin expression in miR-155-KO TA muscles 3 days after CTX injury is normalized when miR-155 mimic was injected compared with control miRNA mimic
