Figure 2
From: Bax/Bak activation in the absence of Bid, Bim, Puma, and p53
Loss of Bid, Bim, and Puma suppresses apoptosis induced by multiple stimuli. (a) The wild-type and the mutant clones of HCT116 were treated with TRAIL (25 ng/ml) for 5 h or with Thapsigargin (3 μM) for 24 h or serum starved for 48 h. Following each treatment, cell lysates were western blotted against an anti-PARP antibody. A representative of three independent experiments is shown. (b) The indicated cells were treated by the same apoptotic stimuli as in (a), and were stained with Annexin V followed by flow cytometry analysis. Each data point is mean +/− S.E.M. from at least three independent experiments
