Figure 2

dMfn mediates the recruitment of defective mitochondria to ER contact points in pink1 or parkin mutants. (a) The RNAi-mediated suppression of dMfn reduces dMfn protein levels in pink1 and parkin mutants. Whole-fly lysates were analysed using the indicated antibodies. (b and c) Quantification of mitochondria–ER contacts in adult Drosophila brains (asterisks, chi-square two-tailed, 95% confidence intervals) (b), and representative electron microscopy images (c). Yellow asterisks show mitochondria in contact with ER (arrows). ER, endoplasmic reticulum; m, mitochondria. Genotypes for (a) Control: daGAL4; pink1^B9: pink1^B9,daGAL4; park²⁵: park²⁵/park²⁵,daGAL4. RNAi dMfn was driven by daGAL4. (b and c) Control: elavGAL4; pink1^B9: pink1^B9,elavGAL4; park²⁵: park²⁵/park²⁵,elavGAL4. RNAi dMfn was driven by elavGAL4