Figure 3
Cell viability assays. (a) sTR, FA1FT, HA5FT and HA5ST mediated cytotoxicity in cancer cell lines: SW480 human colon adenocarcinoma cells, ZR-75-30 human breast cancer cells, Hela human cervical cancer cells and SMMC-7721 hepatocellular carcinoma cells. All cells were incubated for 16 h with the indicated concentrations of proteins in a 96-well plate. (b) Cytotoxicity of sTR, FA1FT, HA5FT and HA5ST in normal human cell lines: Chang liver human hepatocytes and MCF-10A human mammary cells. All cells were incubated for 72 h with the indicated concentrations of proteins. Cell viability was determined using the MTT assay, and results were calculated as a percent relative to untreated cells. Representative killing curves from experiments with n=3 replicates are shown
