Figure 4
In vitro apoptosis activities of sTR, FA1FT, HA5FT and HA5ST. (a) Cells were treated with FA1FT, HA5FT and HA5ST at 10 nM for 6 h. Cells were analyzed using an apoptosis assay kit and flow cytometry. (b) Cells were treated with FA1FT, HA5FT and HA5ST at 10 nM for indicated times. PBS alone at the same time as control. Activities of caspase-3, -8 and -9 were measured by using substrate peptides Ac-DEVD-pNA, Ac-IETD-pNA and Ac-LEHD-pNA, respectively. The release of p-nitroanilide (pNA) was qualified by determining the absorbance at 405 nm, and results were calculated as a percent relative to PBS-treated cells
