Figure 5
Improved characteristics of recombinant proteins in vitro. Killing activities of sTR, FA1FT and HA5ST were analyzed by using ZR-75-30 cells. Recombinant proteins were incubated at (a) 37 °C in PBS, (b) 4 °C in PBS or (c) 37 °C in plasma for the indicated times. Samples were obtained and kept on ice until used in the MTT assay (mean±S.E.M., n=3). (d) The same killing assay was performed as for samples in (a–c), but the proteins were subjected to up to 8 freeze/thaw cycles. Representative killing curves from experiments with n=3 replicates are shown. Statistical analysis was carried out with unpaired t-test, two-tailed test, *P<0.05, **P<0.01, ***P<0.001
