Figure 5
From: ZnCl2 sustains the adriamycin-induced cell death inhibited by high glucose
ZnCl2 cotreatment does not re-establish the ADR-induced cell death, inhibited by HG, in DN-JNK-HA cells. (a) RKO and HCT116 cells were stably transfected with JNK1-APF-HA mutant (dominant negative (DN-JNK-HA)) or with control vector (empty vector) and transfected protein was detected by western immunoblotting with anti-HA antibody. Anti-β-actin was used as protein loading control. The predicted molecular weight is indicated (kDa). (b) Control cells and cells transfected with DN-JNK-HA were treated with ADR (2 μg/ml) in low and high glucose condition with or without ZnCl2 (100 μM) for 24 h, before being assayed for cell viability by Trypan blue exclusion. Data are presented as mean±S.E.M. (n=6) (one-way ANOVA plus Bonferroni test, **P<0.001 ADR (empty vector) versus Mock (empty vector), ADR/HG/ZnCl2 (empty vector) versus ADR/HG (empty vector), ##P<0.001 ADR (DN-JNK) versus Mock (DN-JNK), ADR (DN-JNK) versus ADR (empty vector), #P<0.01 ADR/HG (DN-JNK) versus ADR (DN-JNK), *P<0.01 ADR/HG versus ADR (DN-JNK), not statistically significant (NSS) ADR/HG/ZnCl2 (DN-JNK) versus ADR/HG (DN-JNK)
