Figure 6
From: ZnCl2 sustains the adriamycin-induced cell death inhibited by high glucose
ZnCl2 impairs NF-κB nuclear localization in HG condition. (a) RKO cells were grown in HG condition for 24 h with or without ZnCl2 (100 μM) before being assayed for immunocytochemistry with anti-NF-κB antibody. Nuclei were stained with Hoechst. (b) Western blotting and relative quantification of nuclear NF-κB/Lamin A ratio in RKO cells grown in HG condition for 24 h with or without ZnCl2 (100 μM) and ADR (2 μg/ml) treatment. Data represent mean±S.D. (c) Cells treated as in (a) were assayed for semi-quantitative RT-PCR of Twist-1 gene; 28S was used as a control for efficiency of RNA extraction and transcription. Densitometric analysis for quantification of 28S/Twist-1 ratio is presented as mean±S.E.M. of n=4 (RT-PCR) (one-way ANOVA plus Bonferroni test, *P<0.01 HG versus Mock, HG/ZnCl2 versus HG, HG/ADR/ZnCl2 versus HG/ADR)
