Figure 3
H2O2 changes the levels of miRNAs in CPC-exosomes. (a) Main reported miRNAs participated in cardiomyocytes apoptosis, H2O2 induced apoptosis and miRNAs contained in the EVs after myocardial infarction, some miRNAs are multifunctional in both fields, such as miR-21, miR-214 and miR-34a. (b) Quantitative PCR analysis of main reported miRNAs in Nor-exo and H2O2-exo, six miRNAs (miR-21, 24, 214, 132, 195, 210) are founded in H2O2-exo, and only miR-21 was significantly upregulated (N=6, **P<0.01 versus Nor-exo). (c) RT-qPCR analyzed and miR-21 in H9C2 cells after different concentrations of H2O2 treated (N=3, *P<0.05, **P<0.01 versus 0 μM). (d) 100 μM H2O2-treated H9C2 cells transfected with miR-21 mimics, inhibitors and both negative controls, and western blotting analyzed procaspase-3 and cleaved caspase-3 proteins levels in H9C2 cells (N=5, **P<0.01, ***P<0.001 versus blank group)
