Figure 5

CPC-exosomes inhibit the apoptotic function caused by H₂O₂ in cardiomyocytes. (a) RT-qPCR analyzed miR-21 in H9C2 cells under 100 μM H₂O₂ after CPCs co-culture, Nor-exo and H₂O₂-exo pre-protection (N=4, *P<0.05, ***P<0.001 versus control group). (b) Western blotting analyzed procaspase-3, cleaved caspase-3 and PDCD4 in H9C2 cells under 100 μM H₂O₂ after CPCs co-culture, Nor-exo and H₂O₂-exo pre-protection (N=4, **P<0.01, ***P<0.001 versus control groups). (c–g) Representative dot plots of cell apoptosis were showed after Annexin V/PI dual staining. (h) The percentage of apoptotic cells was represent for both early and late apoptotic cells (N=3, *P<0.05, **P<0.01 versus H₂O₂ groups)