Figure 2

MDM2 is needed for STAT activation. (a) Visualization of MS-based SILAC ratios for known STAT targets. Yellow-colored nodes are upregulated in p53^−/− MEFs. (b) p53^−/− and p53^−/−;mdm2^−/− MEFs were treated with the JAK inhibitor, P6. Protein and phosphorylation levels of JAKs and the adipogenic STATs were measured using western blotting. (c and d) 3T3-L1 preadipocytes were treated with P6 and/or forskolin. (c) Western blot analyses of protein and phosphorylation levels of the proadipogenic STATs. (d) mRNA levels of Cebpb and Cebpd as assessed by real-time qPCR. (e and f) P6 or vehicle was included during adipogenesis of 3T3-L1 cells. Levels of differentiation were scored by Oil-Red-O staining of triglycerides (e) or mRNA levels of adipocyte marker genes by real-time qPCR (f). *Significance tested using Student’s t-test, P<0.05