Figure 1

GPR177 protein level and localisation in mouse testes. (a) Expression of GPR177 in mouse tissues, including spleen, lung, kidney, thymus, stomach, brain and testes by western blot. (b) Expression of GPR177 in testes at different developmental stages, including E15.5, PND 3, 7, 14, 21, 28 and 56. (c) Protein level of GPR177 in W/W^v testes, W/W^v recipient testes after SSC transplantation, and isolated Sertoli, germ and interstitial cells. β-Tubulin served as a protein loading control in (a–c). (d) The cellular localisation of GPR177 in sections of testes from E15.5 and PND56 mice was displayed by immunofluorescent staining. The nuclei are counterstained with DAPI (blue). The images shown were representative results of experiments that were repeated three times using samples from different sets of testes, which yielded similar results. Scale bars, 50 μm