Figure 2 | Cell Death & Disease

Figure 2

From: Does murine spermatogenesis require WNT signalling? A lesson from Gpr177 conditional knockout mouse models

Figure 2

Targeted disruption of the Gpr177 gene. (a–c) Hybrid scheme used to develop Gpr177 cKO mice. Mice carrying a targeted Gpr177 allele (LoxP sites flank exon 3 of the Gpr177 allele) were crossed with Mvh-Cre or Stra8-Cre or Amh-Cre transgenic mice to selectively delete Gpr177. The gene knockout was confirmed by PCR genotyping. The genomic DNA isolated from the mouse tails was amplified with primer pairs specific for the wild-type (+) (~100 bp) and flox alleles (~200 bp) or different Cre bands (Mvh-Cre: 240 bp; Stra8-Cre: 326 bp and Amh-Cre: ~100 bp). (d–f) qRT-PCR analysis showing the conditional loss of Gpr177 mRNA in total testis extracts, germ cell or Sertoli cell extracts of three Gpr177 cKO mice. Gapdh served as the internal control gene. The data are expressed as the mean±S.E.M. *P<0.05, **P<0.01

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