Figure 6
Monoamines and exogenously administered SPM disrupt normal NET structure. (a) Representative images of NET structures generated with PMA alone (I), or in the presence of MPNH2 (II), PNH2 (III), MDC (IV), BPNH2 (V) or SPM (VI) for 4 h. DNA was stained with SytoxOrange (red). The experiment was repeated at least four times with neutrophils from independent healthy donors, with similar results. The original magnification was × 60, the scale bar represents 10 μm. (b) Quantitative evaluation of different parameters of the NET. The average length (I) width (II) of the NET strands, number of junctions per unit strand length (III) and the orientation of the DNA filament (IV) were quantified from control, BPNH2 and MDC treated NETs derived from four independent donors. At least 10 independent fields of view from each sample (donor and treatment) were used for the calculation. Data are presented with box and whisker plots and Welch's t-test was used for testing the equality of the means of any two samples. P-values are summarized in chart
