Figure 3

c-Kit⁺ cardiac cell distribution is not affected by c-Kit^TgD814Y mutation in vivo. (a) Immunofluorescence on heart sections prepared from 1 dpp, 10 dpp and adult c-Kit^TgD814YpKit^EGFP mice showing intramyocardial (1 dpp and Adult) and peri-vasal (10 dpp) c-Kit⁺ cells localization. Insets show c-Kit (red) and EGFP (green) co-expression. Scale bars 30 μM. (b) Count of EGFP⁺ cells in c-Kit^TgD814YpKit^EGFP versus pKit^EGFPmice at 1 dpp, 10 dpp and Adult. At least 20 sections were analyzed for each heart (n=3 mice for each age and genotype). The entire heart was sectioned and analyzed for 1 dpp mice. Data are reported±S.E.; *P<0.05