Figure 6 | Cell Death & Disease

Figure 6

From: Inhibitor of apoptosis protein expression in glioblastomas and their in vitro and in vivo targeting by SMAC mimetic GDC-0152

Figure 6

Multiple reaction monitoring (MRM) chromatograms of FFPE tissue extracts from control mouse brain (upper) and GDC-0152-treated mouse brain (lower). Linearity: Standard curve was obtained by linear regression using external calibration with 5 points in the range of 1 to 100 ng/ml. The calibration curve was linear over the range of 1 to 100 ng/ml with a mean correlation coefficient of 0.999. Quantification was achieved using peak area of compounds interpolated from the standard curve. Optimization: Extraction recoveries and feasibility were estimated comparing control FFPE brain tissue sections spiked with 5 μl of 50 ng/ml of GDC-0152 and control sections spiked with just the solvent. Detection of GDC-0152 in treated FFPE mouse brain sections. The limit of detection corresponds to a signal to noise (S/N) ratio of ⩾3. The result obtained shows a detection of GDC-0152 in treated brains compared with control

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