Table 2 Temporal cytokine/chemokine profiles in distal colon of GrzM-deficient mice during experimental DSS-induced UC

From: Granzyme M has a critical role in providing innate immune protection in ulcerative colitis

 

D0

D1

D3

D5

D7

GM-CSF

nd

nd

nd

nd

nd

G-CSF

nd

nd

nd

ns

↑↑↑

IFN-γ

ns

↓↓↓

ns

ns

ns

IL-1α

nd

nd

nd

ns

↑↑↑

IL-1β

nd

nd

nd

ns

↑↑↑

IL-2

nd

nd

nd

nd

nd

IL-6

nd

nd

ns

ns

ns

IL-10

nd

nd

nd

nd

ns

IL-12p70

ns

ns

ns

ns

ns

IL-13

nd

nd

nd

nd

nd

IL-17A

nd

nd

nd

↑

nd

IL-21

ns

ns

ns

ns

ns

IL-22

ns

ns

ns

ns

ns

IL-23

ns

ns

↓↓

ns

ns

IL-28A/B

ns

ns

ns

ns

ns

IP-10

ns

↓

ns

ns

ns

KC

nd

nd

nd

ns

ns

MCP-1

nd

nd

nd

nd

ns

MIP-1α

nd

nd

nd

nd

↑↑↑

MIP-1β

nd

nd

nd

ns

↑↑↑

RANTES

ns

ns

ns

↑

ns

TGF-β1

ns

ns

ns

ns

ns

TNFα

nd

nd

nd

↑

ns

  1. Abbreviations: nd, not detected; ns, not significant. Distal part of colons of WT or GrzM-deficient mice was harvested in different time points after 5% DSS challenge (days 0, 1, 3, 5, and 7), homogenized in tissue protein extraction buffer, and analyzed for the indicated cytokines/chemokines. Pool of two independent experiments of n=5 mice each (total n=10). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test, where *P<0.05 (↑ for significantly higher, or ↓ for significantly lower than WT), **P<0.01 (↑↑ for significantly higher, or ↓↓ for significantly lower than WT), and ***P<0.001 (↑↑↑ for significantly higher, or ↓↓↓ for significantly lower than WT) were considered for statistical significance.
  2. • Pro-inflammatory neutrophil-related cytokines/chemokines analyzed: GM-CSF, G-CSF, IL-17A, and KC.
  3. • Chemokines analyzed: IP-10, MCP-1, MIP-1α, MIP-1β, and RANTES.
  4. • Pro-inflammatory cytokines analyzed: IFN-γ, IL-1α, IL-1β, IL-2, IL-6, IL-12, IL-13, IL-21, IL-23, IL-28A/B, and TNFα.
  5. • Anti-inflammatory, tissue repair proteins: IL-10, IL-22, and TGF-β1.
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