Figure 3 | Cell Death & Disease

Figure 3

From: Exosome-mediated delivery of miR-9 induces cancer-associated fibroblast-like properties in human breast fibroblasts

Figure 3

MiR-9 is delivered to NFs via exosomes and promotes cell motility. (a and b) qRT-PCR analysis to evaluate miR-9 level in MDA-MB-231 transiently transfected with control or miR-9 and in exosomes purified from tumor cell supernatants, respectively. (c) MDA-MB-231-secreted exosomes were fed on NFs for 72 h, then RNA was extracted from the recipient cells and analyzed for miR-9 level by qRT-PCR. The data are shown as normalized relative to miR-21 (exosomes) or RNU44 (MDA-MB-231 and NFs, respectively) (***P<0.0005). (d) Migration by transwell (left panel) and invasion assays (right panel) of recipient NFs after miR-9 internalization. Quantitative analysis of the experiments was shown in the lower histograms. Data are presented as the mean±S.D. of three views (*P<0.05). Scale bars, 100 μm

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