Figure 4
Analysis of cell death events in AOM/DSS-induced tumors. (a) H&E staining (left column), identifying gathered neoplastic lesions as well-differentiated adenocarcinomas. IHC staining against Bcl-xL and cleaved PARP, revealing remarkable amounts of apoptotic cells in Bcl-xL-negative but not control tumors. (b) Immunoblotting and subsequent densitometric analysis, showing a 3.2-fold upregulation of cleaved Caspase 8 (P=0.035) and a 2.3-fold upregulation of cleaved Caspase 9 (P=0.045) in Bcl-xL-negative tumors compared with controls. Expression levels of Mcl-1 and Bcl-2 remain unaltered. Values are expressed as means+S.D. *P<0.05
