Figure 2 | Cell Death & Disease

Figure 2

From: Ginsenoside compound K sensitizes human colon cancer cells to TRAIL-induced apoptosis via autophagy-dependent and -independent DR5 upregulation

Figure 2

CK sensitized TRAIL-resistant HT-29 cells to TRAIL. HT-29 cells were pretreated with or without 20 μM z-VAD-fmk for 1 h, then treated with indicated doses of CK for 24 h and co-treated with or without TRAIL (100 ng/ml) for 24 h. Cell viability was analyzed using the WST-1 assay (a) and crystal violet staining assay (b). Cell apoptosis (as indicated by sub-G1 DNA content to the left of the G1 peak) was tested by flow cytometry followed by PI staining (c), the processing of the caspases was tested by western blot followed by pretreated with or without 20 μM z-VAD-fmk for 1 h, and then treated with CK (25 μM; 48 h) and TRAIL (100 ng/ml; 24 h). Actin was used as a protein loading control (d). Error bars in (a) and (c) represent the S.D. (N=3 independent experiments). *P<0.05, **P<0.01 compared with DMSO group (Student’s t-test, two tailed)

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