Figure 5 | Cell Death & Disease

Figure 5

From: Ginsenoside compound K sensitizes human colon cancer cells to TRAIL-induced apoptosis via autophagy-dependent and -independent DR5 upregulation

Figure 5

JNK kinase expression had a crucial role in autophagy-mediated DR5 expression. HCT116 cells were pretreated with (b) or without (a) the JNK inhibitor SP600125 or transfected with JNK siRNAs (d) and then exposed to CK at the indicated doses. Whole-cell extracts were prepared and analyzed by western blotting using protein-specific antibodies. HCT116 cells were pretreated with SP600125 for for 1 h and then treated with 50 μM CK for 24 h and then co-treated with or without 25 ng/ml TRAIL for 24 h (c). Cell death (as indicated by sub-G1 DNA content to the left of the G1 peak) was measured by FACS. Error bars in (c) represent the S.D. (N=3 independent experiments). *P<0.05 compared with DMSO group (Student’s t-test, two tailed)

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