Figure 7

CHOP and p53 influenced upregulation of DR5. HCT116 cells were pretreated with CK at the indicated doses for 24 h or with CK at 50 μM for the times indicated. Whole-cell extracts were prepared and analyzed by western blotting (a). HCT116 cells were transfected with control siRNA or CHOP siRNA and then exposed to 50 μM CK for 24 h. Whole-cell extracts were prepared and analyzed by western blotting (b). HCT116 cells were transfected with control siRNA or p53 siRNAs (c) and were treated with CK at the doses indicated. Whole-cell extracts were then prepared and analyzed by western blotting