Figure 3
From: Golgi-associated LC3 lipidation requires V-ATPase in noncanonical autophagy
AMDE-1-induced NCA does not recruit WIPI2, but Atg16 and Atg12. (a) WT-MEFs and FIP200KO-MEFs were treated with or without AMDE-1 (10 μM) or rapamycin (Rap, 1 μM) for 6 h and then assessed for WIPI2 staining in the cytosol (Red). (b and c) WT-MEFs and FIP200KO-MEFs were treated as indicated (Rap, 1 μM; AMDE-1, 10 μM; 3-MA, 10 mM) for 6 h, followed by immunostaining of ATG16 (b) and ATG12 (c). Arrows indicate the examples of WIPI2-, ATG12- and ATG16-positive dots. (d) Costaining of ATG12 (green) and ATG16 (red) in FIP200KO-MEF after AMDE-1 treatment for 6 h. Arrowheads indicate the examples of colocalized dots of ATG12 and ATG16. Bar=25 μm
