Figure 5
From: Golgi-associated LC3 lipidation requires V-ATPase in noncanonical autophagy
AMDE-1-induced NCA involves V-ATPase, but not lysosome activity or intracellular ion redistribution. (a–c) FIP200KO-MEFs were treated with AMDE-1 (10 μM) in the presence or absence of Baf, ConA or SalA for 6 h, followed by immunoblotting analysis for LC3. (d–g) FIP200KO-MEFs were treated as indicated for 6 h, followed by immunoblotting analysis (d, f and g) and ATG12 immunostaining (e). AMDE-1 (10 μM), Baf (0.5 μM), AC (20 mM), CQ (40 μM), E64d (25 μM) plus pepstatin A (50 μM), monesin (Mon, 10 μM), nigarecin (Nig, 1.5 μM), gramicidin A (Gra, 5 μM), valinomycin (Val, 10 μM), ConA (0.2 μM), ionomycin (Iono, 2 μM) and A23187 (2 μM). Bar=25 μm
