Figure 8

AMDE-1-induced NCA can be blocked by Golgi toxins. (a) FIP200KO-MEFs were treated by AMDE-1 (10 μM) with or without BFA (5 μg/ml) for 6 h, followed by immunostaining for LC3 and GM130. Arrows indicate colocalization of LC3 with GM130. (b). FIP200KO-MEFs expressing GFP-LC3 were treated by AMDE-1 (10 μM) with or without BFA (5 μg/ml) for 6 h, followed by immunostaining of SQSTM1. Arrows indicate colocalization of GFP-LC3 with SQSTM1. (c and d) FIP200KO-MEFs were treated by AMDE-1 (10 μM) with or without BFA (5 μg/ml) or GCA (20 μM) for 6 h, followed by immunoblotting analysis. (e–k) Immunoblotting analysis of FIP200KO-MEFs treated with CQ (100 μM, 6 h, e), Ole (500 μM, 6 h, f), AC (10 mM, 24 h, g), hypotonic buffer (Hypo, 1 h, h), BZ (30 nM, 12 h, i), MG132 (MG, 5 μM, 12 h, j), and GO (20 μM, 12 h, k) with or without GCA (20 μM). Bar=25 μm