Figure 7
From: MiR-182-5p protects inner ear hair cells from cisplatin-induced apoptosis by inhibiting FOXO3a
MiR-182 attenuated the synergetic effect of PI3K inhibitor and cisplatin on hair cell loss. (a) The synergetic effect of PI3K inhibitor and cisplatin on hair cell loss and the protective effect of miR-182. Loss of hair cells was significantly exacerbated when organ of Corti explants were treated with LY294002 and cisplatin in combination at 24 h. Transfection with miR-182 mimics before LY294002 and cisplatin treatment significantly improved hair cell survival. (b) Intense expression of FOXO3a in the hair cell nuclei in cochlear explants treated with cisplatin and LY294002 together (b, upper panel) and control oligo group (b, middle panel), whereas lower fluorescence intensity in hair cell nuclei was observed in miR-182 overexpression group (b, lower panel). Data are shown as means±S.E. One-way ANOVA followed by Newman–Keuls test, **P<0.01. Scale bar in bottom-right corner: 20 μM. ns, no significance
