Figure 6 | Cell Death & Disease

Figure 6

From: Epstein-Barr virus-encoded miR-BART6-3p inhibits cancer cell metastasis and invasion by targeting long non-coding RNA LOC553103

Figure 6

EBV-miR-BART6-3p and LOC55310 regulate EMT and metastasis-related gene expression. (a) 5-8 F, HNE2, AGS or C666-1 cells were transfected with EBV-miR-BART6-3p mimics (BART6-3p) or EBV-miR-BART6-3p inhibitors (BART6-3p In), LOC553103 siRNAs (siLOC) or LOC553103 overexpression vector. Forty-eight hours after transfection, cells were harvested and mRNA expression levels of metastasis-related genes MMP2, MMP9, EMT-related genes CDH1 (encoding E-cadherin protein), CDH2 (encoding N-cadherin) SNAL1 and CTTNB1 (encoding β-catenin) were measured by real-time PCR. (b) Important EMT markers β-catenin, E-cadherin, N-cadherin and Snail protein expression were measured by western blot 48 h after transfection with EBV-miR-BART6-3p mimics (BART6-3p) or EBV-miR-BART6-3p inhibitors (BART6-3p In), LOC553103 siRNAs (siLOC) or LOC553103 overexpression vector in 5-8 F, HNE2, AGS or C666-1 cells

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