Figure 5 | Cell Death & Disease

Figure 5

From: Kinsenoside screening with a microfluidic chip attenuates gouty arthritis through inactivating NF-κB signaling in macrophages and protecting endothelial cells

Figure 5

Effects of Kin on MSU-induced NF-κB/MAPK activation. (a) Transfected RAW264.7 macrophage cells were incubated with Kin and MSU for 7 h. The luciferase activity for NF-κB was evaluated and normalized to the control. (b) Expression of NF-κB signaling-associated upstream proteins (TRAF-6, p-TAK-1/TAK-1, p-IKKα β/IKKαβ) were compared in macrophages treated with MSU crystals and Kin. (c) Expression of NF-κB signaling-associated downstream proteins (p-IκBα/IκBα, p-p65/p65) was compared in macrophages treated with MSU crystals and Kin. (d) Molecular docking of IKKα and IKKβ with Kin. (e) Expressions of MAPK signaling-associated proteins (p-JNK/JNK, p-Erk/Erk, p38/p-p38) were compared in macrophages treated with MSU crystals and Kin. The data are presented as the means±S.D. **P<0.05 compared with 0 μg/ml of Kin. #P<0.05 compared with control. All data were obtained from at least three independent experiments

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