Figure 6 | Cell Death & Disease

Figure 6

From: Kinsenoside screening with a microfluidic chip attenuates gouty arthritis through inactivating NF-κB signaling in macrophages and protecting endothelial cells

Figure 6

Effects of Kin on the expression of MSU-induced NF-κB downstream inflammatory cytokines and inflammasome and schematic diagram of the proposed mechanisms of antigouty arthritis through modulation of macrophages. (A) mRNA expressions of NF-κB downstream IL-1β, IL-6, TNF-α, NO and PGE2 in macrophages treated with MSU crystals and Kin. (B) Measurement of the secretion of NF-κB downstream IL-1β, IL-6, TNF-α, NO and PGE2 in macrophages treated with MSU crystals and Kin. (C) Expressions of NALP3 and TLR2 were compared in macrophages treated with MSU crystals and Kin. (D) Effects of Kin on MSU-stimulated expression of COX-1 in HUVECs and COX-2 in inflammatory macrophages. (E) Kin inactivates NF-κB pathway via targeting of IKK kinases, abrogating phosphorylation of IκBα and p65, downregulating expression of IL-1β, IL-6, TNF-α, NO and PGE2 in macrophages, attenuating MSU-mediated HUVECs impairment. The data are presented as the means±S.D. **P<0.05 compared with 0 μg/ml of Kin. #P<0.05 compared with control. All data were obtained from at least three independent experiments

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