Figure 2 | Cell Death & Disease

Figure 2

From: miR-150 exerts antileukemia activity in vitro and in vivo through regulating genes in multiple pathways

Figure 2

miR-150 suppresses cell proliferation and induces apoptosis in leukemia cell lines and primary AML samples. (a) miR-150 expression in leukemia cell lines and in the cell lines infected with miR-150. (b) Cell growth curves of K562, Kasumi-1, and THP-1 cells infected in vitro with miR-150, and their respective controls. (c) Caspase-3 activation in leukemia cell lines 48 h after miR-150 or empty vector transfection determined by immunoblotting. (d) Annexin V/7-AAD assays in leukemia cell lines 48 h after miR-150 or empty vector transfection. The results are presented as percentage of apoptotic cells. (e) Apoptosis analysis in two primary AML samples after 48 h of transfection with miR-150 or empty vector in the presence or absence of 5 μM of Ara-C. Data are mean±S.D. of triplicates assays. *P<0.05; **P<0.01; ***P<0.001; #, no significance

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