Figure 7
Knockdown of miR-150 targets (EIF4B, FOXO4, PRKCA, and TET3) inhibits leukemia cell growth. (a) Western blot analyses showing the efficiency of shRNA for EIF4B, FOXO4, PRKCA, and TET3, respectively. β-Actin was used as the loading control. (b) Cell growth curves of K562 cells infected in vitro with miR-150 or shRNA against its targets indicated. (c) Apoptosis activation measuring by the level of cleaved caspase-3 with western blot. (d) Representative images and statistics of soft agar colony formation assay. K562 cells were transfected with the indicated shRNA or an empty vector as control. Cells were then cultured in methylcellulose. Colonies were counted after 14 days. All data are represented as mean values±S.D. (n=3). ***P<0.001 for each shRNA group compared with control
