Figure 5
Effects of siRNA-mediated survivin depletion on neuroblastoma cell viability. (a) Western blots indicating levels of γH2AX, H2AX, phosphorylated Chk2 (pChk2), Chk2, cleaved PARP, Mcl-1, cIAP-1, cIAP-2, XIAP, or survivin in neuroblastoma cells, neuroblastoma cells transfected with non-targeting control siRNA, and neuroblastoma cells transfected with siRNA targeting survivin (BIRC5) 24 h post transfection. β-actin served as loading control. (b) Levels of survivin and γH2AX (indicating DNA damage) in UKF-NB-3 cells transduced with a lentiviral vector encoding for shRNA targeting p53 (UKF-NB-3p53shRNA) in response to YM155 treatment for 48 h as indicated by quantitative, fluorescence-based western blot analysis; values are presented as fold change relative to the respective parental cell line. Representative western blots are provided in Supplementary Figure 4. (c) Viability of UKF-NB-3 cells, UKF-NB-3rVCR10 cells, and UKF-NB-3p53shRNA cells 48 h after transfection with siRNA targeting survivin (BIRC5); Western blots indicating depletion efficiency in UKF-NB-3rVCR10 cells and UKF-NB-3p53shRNA cells are presented in Supplementary Figure 11. Numerical values including controls transfected with non-targeting siRNA are shown in Supplementary Table 5
