Figure 6
Role of survivin in response to irradiation-induced DNA damage in neuroblastoma cells. (a) Representative western blots indicating cellular levels of γH2AX and survivin 24 h after irradiation of UKF-NB-3 cells, UKF-NB-3 cells transduced with a lentiviral vector encoding shRNA directed against p53 (UKF-NB-3p53shRNA), or UKF-NB-3 cells transduced with a control vector encoding non-targeting (‘scrambled’) shRNA (UKF-NB-3scrshRNA). β-actin served as a loading control. Quantitative, fluorescence-based western blot analyses are presented in Supplementary Figure 6. (b) Viability of UKF-NB-3, UKF-NB-3p53shRNA, or UKF-NB-3scrshRNA cells 24 h post irradiation as indicated by MTT assay; *P 0.05 relative to untreated control cells; (c) Combined effects of irradiation and YM155 on UKF-NB-3 (1 Gy, YM155 0.625 nM), UKF-NB-3p53shRNA (3 Gy, YM155 2.5 nM), or UKF-NB-3scrshRNA (1 Gy, YM155 0.625 nM) cell viability 24 h post irradiation as indicated by MTT assay; *P 0.05 relative to either single treatment
